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KMID : 0364820130490040336
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Korean Journal of Microbiology
2013 Volume.49 No. 4 p.336 ~ p.342
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Microbial Diversity of the Trichloroethylene Contaminated Groundwater Treatment System and Characterization of Pseudomonas sp. DHC8
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Nam Ji-Hyun
Shin Ji-Hye
Kwon Ki-Wook
Bae Woo-Keun
Lee Dong-Hun
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Abstract
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Trichloroethylene (TCE) is a widely used substance in commercial and industrial applications, yet it must be
removed from the contaminated soil and groundwater environment due to its toxic and carcinogenic nature. We
investigated bacterial community structure, dominant bacterial strain, and removal efficiency in a TCE contaminated
groundwater treatment system using immobilized carrier. The microbial diversity was determined by the nucleotide
sequences of 16S rRNA gene library. The major bacterial population of the contaminated groundwater treatment
system was belonging to BTEX degradation bacteria. The bacterial community consisted mainly of one genus of
Pseudomonas (Pseudomonas putida group). The domination of Pseudomonas putida group may be caused by high
concentration of toluene and TCE. Furthermore, we isolated a toluene and TCE degrading bacterium, named
Pseudomonas sp. DHC8, from the immobilized carrier in bioreactor which was designed to remove TCE from the
contaminated ground water. Based on the results of morphological and physiological characteristics, and 16S rRNA
gene sequence analysis, strain DHC8 was identified as a member of Pseudomonas putida group. When TCE (0.83
mg/L) and toluene (60.61 mg/L) were degraded by this strain, removal efficiencies were 72.3% and 100% for 12.5 h,
respectively. Toluene removal rate was 2.89 μmol/g-DCW/h and TCE removal rate was 0.02 μmol/g-DCW/h. These
findings will be helpful for maintaining maximum TCE removal efficiency of a reactor for bioremediation of TCE.
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KEYWORD
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Pseudomonas sp. DHC8, immobilized bacterial carrier, microbial diversity, TCE contaminated groundwater
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